Commit e8ffad7e authored by Lucy McNeill's avatar Lucy McNeill
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update vignette to emphasise that count_foci and measure_distances_general...

update vignette to emphasise that count_foci and measure_distances_general find the coincident foci mask with the exact same input parameters, and it is much faster to play around with/ calibrate your input parameters with count_foci, since at the moment measure_distances_general is relatively very slow
parent 5dda3c25
......@@ -145,6 +145,8 @@ foci_counts <- count_foci(path,offset_factor = 5, brush_size = 1, brush_sigma =
```
foci_counts is a data frame summarizing some features (including foci counts) of the cells classified as pachytene.
**note: count_foci and measure_distances_general take in the same arguments used to create the coincident foci mask. To save time, find the optimum settings for e.g. offset_factor, brush_size, brush_sigma, watershed_stop etc. with count_foci (a much faster function). When you are confident that these settings are identifying the correct amount of foci per cell, run measure_distances_general (slower)**
## Distance between foci on SC
```{r}
df_dist <- measure_distances_general(path,offset_factor = 5, brush_size = 1, brush_sigma = 1, annotation = "on", stage = "pachytene",target_foci_number = 2)
......
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