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Lucy McNeill
synapsis
Commits
74164285
Commit
74164285
authored
3 years ago
by
Lucy McNeill
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include figures in overview
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README.md
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74164285
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@@ -28,23 +28,23 @@ We summarise them in the following subsections:
### auto_crop
input:
input:
Original grey scale image files of (1) Synaptonemal complexes (e.g. SYCP3 anti-body) and (2) Foci (e.g. MLH1, MLH3 anti-body) channels from e.g. Nikon .nd2 files.
output:
output:
crops in channels (1) '
*dna.jpeg' and (2) '*
foci.jpeg' around individual cells.

### get_pachytene
input:
input:
crops in channels (1) '
*dna.jpeg' and (2) '*
foci.jpeg' around individual cells, from previous auto_crop.
output:
output:
only keeps crops if cells are in pachytene phase (based on channel (1))
### count_foci
input:
input:
crops of dna and foci channels in pachytene phase (from get_pachytene)
output:
output:
number of foci counts of synamtonemal complexes per cell (i.e. channel 1 coincident with channel 2) as a function of genotype.
### measure_distances
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@@ -54,6 +54,14 @@ output:
## Analysis




## Project organisation and management
Please issue bug reports through GitLab.
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