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Commit 74164285 authored by Lucy McNeill's avatar Lucy McNeill
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include figures in overview

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......@@ -28,23 +28,23 @@ We summarise them in the following subsections:
### auto_crop
input:
input: Original grey scale image files of (1) Synaptonemal complexes (e.g. SYCP3 anti-body) and (2) Foci (e.g. MLH1, MLH3 anti-body) channels from e.g. Nikon .nd2 files.
output:
output: crops in channels (1) '*dna.jpeg' and (2) '*foci.jpeg' around individual cells.
![cropping](resources/figures/cropping_procedure.png)
### get_pachytene
input:
input: crops in channels (1) '*dna.jpeg' and (2) '*foci.jpeg' around individual cells, from previous auto_crop.
output:
output: only keeps crops if cells are in pachytene phase (based on channel (1))
### count_foci
input:
input: crops of dna and foci channels in pachytene phase (from get_pachytene)
output:
output: number of foci counts of synamtonemal complexes per cell (i.e. channel 1 coincident with channel 2) as a function of genotype.
### measure_distances
......@@ -54,6 +54,14 @@ output:
## Analysis
![cropping-hist](output/count_foci_histogram.png)
![cropping-box](output/count_foci_boxplot.png)
![distance-hist](output/measure_distances_histogram.png)
![distance-box](output/measure_distances_boxplot.png)
## Project organisation and management
Please issue bug reports through GitLab.
......
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