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Lucy McNeill
synapsis
Commits
17931ecb
Commit
17931ecb
authored
Jul 12, 2021
by
Lucy McNeill
Browse files
update README.md to reflect new crowded_foci option
parent
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...
...
@@ -28,22 +28,22 @@ if you are looking at mouse pachytene (what synapsis is designed for), the follo
```
r
SYCP3_stats
<-
get_pachytene
(
path
,
ecc_thresh
=
0.8
,
area_thresh
=
0.04
)
foci_counts
<-
count_foci
(
path
,
offset_factor
=
5
,
brush_size
=
1
,
brush_sigma
=
1
,
annotation
=
"on"
,
WT_out
=
"Fancm+/+"
,
KO_out
=
"Fancm-/-"
)
foci_counts
<-
count_foci
(
path
,
offset_factor
=
5
,
brush_size
=
1
,
brush_sigma
=
1
,
annotation
=
"on"
,
WT_out
=
"Fancm+/+"
,
KO_out
=
"Fancm-/-"
,
stage
=
"pachytene"
)
```
and
```
r
df_dist
<-
measure_distances
(
path
,
offset_factor
=
5
,
brush_size
=
1
,
brush_sigma
=
1
,
annotation
=
"on"
,
WT_out
=
"Fancm+/+"
,
KO_out
=
"Fancm-/-"
)
df_dist
<-
measure_distances
(
path
,
offset_factor
=
5
,
brush_size
=
1
,
brush_sigma
=
1
,
annotation
=
"on"
,
WT_out
=
"Fancm+/+"
,
KO_out
=
"Fancm-/-"
,
stage
=
"pachytene"
)
```
otherwise, for everything else, you could call something like:
```
r
foci_counts
<-
count_foci
(
path
,
offset_factor
=
5
,
brush_size
=
1
,
brush_sigma
=
1
,
annotation
=
"on"
,
WT_str
=
"tif"
,
WT_out
=
"NA"
,
file_ext
=
"tif"
,
channel2_string
=
"c3"
,
channel1_string
=
"c2"
,
offset_px
=
0.4
)
foci_counts
<-
count_foci
(
path
,
offset_factor
=
5
,
brush_size
=
1
,
brush_sigma
=
1
,
annotation
=
"on"
,
WT_str
=
"tif"
,
WT_out
=
"NA"
,
file_ext
=
"tif"
,
channel2_string
=
"c3"
,
channel1_string
=
"c2"
,
offset_px
=
0.4
,
crowded_foci
=
TRUE
)
```
and
```
r
df_dist
<-
measure_distances
(
path
,
offset_factor
=
5
,
brush_size
=
1
,
brush_sigma
=
1
,
annotation
=
"on"
,
WT_str
=
"tif"
,
WT_out
=
"NA"
,
file_ext
=
"tif"
,
channel2_string
=
"c3"
,
channel1_string
=
"c2"
,
offset_px
=
0.4
)
df_dist
<-
measure_distances
(
path
,
offset_factor
=
5
,
brush_size
=
1
,
brush_sigma
=
1
,
annotation
=
"on"
,
WT_str
=
"tif"
,
WT_out
=
"NA"
,
file_ext
=
"tif"
,
channel2_string
=
"c3"
,
channel1_string
=
"c2"
,
offset_px
=
0.4
,
crowded_foci
=
TRUE
)
```
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