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BioCellGen-public
sscocaller
Commits
09582ce8
Commit
09582ce8
authored
4 years ago
by
Ruqian Lyu
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Merge branch 'master' of gitlab.svi.edu.au:biocellgen-public/sscocaller
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09582ce8
## sscocaller: Calling crossovers from single-sperm DNA sequencing reads
It takes the large bam file which contains aligned DNA reads from a list of single sperm cells and summarizes allele
counts for informative SNP markers. A HMM model is applied for haplotypin each sperm and viterbi algorithm is run
counts for informative SNP markers. A HMM model is applied for haplotypin
g
each sperm and viterbi algorithm is run
for deriving the inferred haplotype sequence against the list of SNP markers.
## Inputs
...
...
@@ -39,3 +39,31 @@ Options:
Examples
./sscocaller --threads 10 AAAGTAGCACGTCTCT-1.raw.bam AAAGTAGCACGTCTCT-1.raw.bam.dp3.alt.vcf.gz barcodeFile.tsv ./percell/ccsnp-
```
## Setup/installation
`sscocaller`
uses
`hts-nim`
(https://github.com/brentp/hts-nim) that requires the
`hts-lib`
library. If you are building the
`sscocaller`
from
source, you would need to install
`hts-lib`
```
git clone --recursive https://github.com/samtools/htslib.git
cd htslib && git checkout 1.10 && autoheader && autoconf && ./configure --enable-libcurl
cd ..
make -j 4 -C htslib
export LD_LIBRARY_PATH=$HOME/htslib
ls -lh $HOME/htslib/*.so
```
Then,
`sscocaller`
can be installed using
`nimble`
### Install with nimble
`nimble install https://gitlab.svi.edu.au/biocellgen-public/sscocaller.git`
The built binary in $HOME/.nimble/bin/sscocaller
### Static builds
The static bianry can be simply downloaded which works for GNU/Linux type OS:
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