diff --git a/course_files/exprs-norm-reads.Rmd b/course_files/exprs-norm-reads.Rmd index ac95675fa5d621ec323910ac887a784bfac0f603..070d805f87e73f0cca0199c619d21166f6fc2411 100644 --- a/course_files/exprs-norm-reads.Rmd +++ b/course_files/exprs-norm-reads.Rmd @@ -135,7 +135,7 @@ plotRLE( -```{r} +```{r, eval=FALSE} reads.qc <- getBMFeatureAnnos( reads.qc, filters = "ensembl_gene_id", @@ -152,20 +152,20 @@ reads.qc <- getBMFeatureAnnos( ) ``` -```{r} +```{r, eval=FALSE} reads.qc.ann <- reads.qc[!is.na(rowData(reads.qc)$ensembl_gene_id), ] ``` -```{r} +```{r, eval=FALSE} eff_length <- abs(rowData(reads.qc.ann)$end_position - rowData(reads.qc.ann)$start_position) / 1000 ``` -```{r} +```{r, eval=FALSE} tpm(reads.qc.ann) <- log2(calculateTPM(reads.qc.ann, eff_length) + 1) ``` -```{r norm-pca-tpm-reads, fig.cap = "PCA plot of the tung data after TPM normalisation"} +```{r norm-pca-tpm-reads, eval=FALSE, fig.cap = "PCA plot of the tung data after TPM normalisation"} tmp <- runPCA( reads.qc.ann, exprs_values = "tpm", @@ -178,11 +178,11 @@ plotPCA( ) ``` -```{r} +```{r, eval=FALSE} tpm(reads.qc.ann) <- log2(calculateFPKM(reads.qc.ann, eff_length) + 1) ``` -```{r norm-pca-fpkm-reads, fig.cap = "PCA plot of the tung data after FPKM normalisation", eval=FALSE} +```{r norm-pca-fpkm-reads, eval=FALSE, fig.cap = "PCA plot of the tung data after FPKM normalisation", eval=FALSE} tmp <- runPCA( reads.qc.ann, exprs_values = "tpm",